|
KMID : 1098420150230060439
|
|
Korean Journal of Medicinal Crop Science
2015 Volume.23 No. 6 p.439 ~ p.445
|
|
|
Internal Transcribed Spacer Barcoding DNA Region Coupled with High Resolution Melting Analysis for Authentication of Panax Species)
|
|
Bang Kyong-Hwan
Kim Young-Chang
Lim Ji-Young
Kim Jang-Uk
Lee Jung-Woo
Kim Dong-Hwi
Kim Ki-Hong
Jo Ick-Hyun
|
|
|
Abstract
|
|
|
Background : Correct identification of Panax species is important to ensure food quality, safety, authenticity and health for consumers. This paper describes a high resolution melting (HRM) analysis based method using internal transcribed spacer (ITS) and 5.8S ribosomal DNA barcoding regions as target (Bar-HRM) to obtain barcoding information for the major Panax species and to identify the origin of ginseng plant.
Methods and Results : A PCR-based approach, Bar-HRM was developed to discriminate among Panax species. In this study, the ITS1, ITS2, and 5.8S rDNA genes were targeted for testing, since these have been identified as suitable genes for use in the identification of Panax species. The HRM analysis generated cluster patterns that were specific and sensitive enough to detect small sequence differences among the tested Panax species.
Conclusion : The results of this study show that the HRM curve analysis of the ITS regions and 5.8S rDNA sequences is a simple, quick, and reproducible method. It can simultaneously identify three Panax species and screen for variants. Thus, ITS1HRM and5.8SHRM primer sets can be used to distinguish among Panax species.
|
|
|
KEYWORD
|
|
|
Panax Species, High Resolution Melting, ITS Barcoding, Real Time PCR
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|